|
KMID : 0613820080180081159
|
|
Journal of Life Science
2008 Volume.18 No. 8 p.1159 ~ p.1163
|
|
|
Assay of Lipase Activity by the pH-Dependent Fluorescence Change of Fluorescein
|
|
Park Jong-Won
Choi Suk-Jung
|
|
|
Abstract
|
|
|
|
The purpose of this study was to establish a high-throughput assay method capable of estimating specific lipase activity at oil-water interface. The method is based on the fact that fluorescence intensity of fluorescein is affected by pH. The pH-dependence might be used to monitor pH change caused by the release of fatty acid through the action of lipase. Assay was performed by incubating a reaction mixture containing oil emulsion, fluorescein and enzyme and by monitoring fluorescence intensity periodically. Fluorescence intensity decreased linearly with a rate proportional to the enzyme amount. Linear relationship was observed between enzyme amount and reaction rate which was calculated from a graph of fluorescence change against time. The assay was possible at different pH conditions in the range of pH 6.0-8.0.
|
|
|
KEYWORD
|
|
|
Lipase, assay, fluorescein, pH
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|